不同年生野山参中总皂苷和总多糖的含量测定

Determination of Total Saponin and Total Polysaccharide in Wild Ginseng of Different Ages

目的建立测定不同年生野山参中人参总皂苷和总多糖含量的方法。方法采用紫外分光光度法在544nm和492nm波长处分别测定总皂苷及总多糖的含量。结果不同年生野山参中人参总皂苷及总多糖的含量分别为3.24%~6.37%、17.50%~22.92%。结论总皂苷及多糖作为野山参中的主要功效成分,含量较高,可以作为野山参质量评定的依据之一。本文的研究为野山参的基础成分研究提供了参考依据,为野山参的质量控制奠定了基础。

Objective To determine the content of total saponin and total polysaccharide in Wild Ginseng of different ages. Methods The content of total saponin and total polysaccharide were determined by ultraviolet spectrophotometry at the wavelength of 544 nm and 492 nm, respectively. Results The content of total saponin were in the range of 3.24% and 6.37%. The content of total polysaccharide ranged from 17.50% to 22.92%. Conclusion Total saponin and total polysaccharide, the main active constituents in Wild Ginseng, were of high levels, which can be considered as one of the reference of quality evaluation. The results in this paper provided useful data for study of the basic constituents and laid foundation for the quality control of Wild Ginseng.