高蛋白、高脂肪类人参食品中人参皂苷的检测方法研究

Study on detection methods of ginsenosides in high protein and high fat ginseng foods

目的通过对比不同前处理方法提取出人参食品中人参皂苷Rg1、Re、Rb1的含量,建立一种操作性强,准确检测高蛋白、高脂肪类人参食品中人参皂苷的含量的方法。方法采用乙醇提取-大孔树脂法与传统药典法进行对比,并对样品中人参皂苷Rg1、Re、Rb1含量及加样回收率进行测定。结果乙醇提取-大孔树脂法提取出皂苷含量约是药典法的3倍,且加样回收率可达到85%以上。结论上述方法可操作性强、稳定性好,适用于高蛋白、高脂肪人参食品中人参皂苷的检验。

Objective: The content of ginsenoside Rg1, Re and Rb1 in ginseng food was extracted by comparing different pretreatment methods, and a method for detecting the content of ginsenoside in high protein and high fat ginseng food was established. Method: The ethanol extraction-macroporous resin method was compared with the traditional pharmacopoeia method, and the content of ginsenoside Rg1, Re, Rb1 and the recovery rate of the sample were determined. Results: The content of Saponins Extracted by ethanol extraction-macroporous resin method is about three times as much as that by pharmacopoeia method, and the recovery rate can reach more than 85%.Conclusion: The above method is operability and stability, and is suitable for the detection of ginsenosides in high protein and high fat ginseng foods.