三阴性乳腺癌中AQP8的表达及其与血管生成、增殖和预后的相关性

Expression of AQP8 in triple negative breast cancer and its correlation with angiogenesis,proliferation and prognosis

目的探讨水通道蛋白8(AQP8)在三阴性乳腺癌(TNBC)中的表达及其与血管生成、增殖和预后的关系。方法通过免疫组织化学法在2010年1月至2013年1月在首都医科大学附属复兴医院接受TNBC根治性手术治疗的127例患者的肿瘤和癌旁组织石蜡切片中检测AQP8的表达情况。同时,采用免疫组织化学法检测TNBC组织中血管内皮生长因子(VEGF)和Ki-67的表达,分析TNBC组织中VEGF、Ki-67的表达与AQP8的关系。结果与癌旁组织相比,TNBC组织中AQP8表达显著增加。这种现象伴随着Ki-67和血管生成相关标志物VEGF的异常表达。此外,AQP8高表达与侵袭性临床病理因素、低总体存活时间(OS)和低无病生存时间(DFS)相关。多变量分析结果显示,AQP8表达可能是TNBC患者低OS、DFS的独立预测因子。结论AQP8高表达与TNBC患者的血管生成、增殖和预后不良有关。

Objective To investigate the expression of aquaporin 8(AQP8)in triple negative breast cancer(TNBC)and its relationship with angiogenesis,proliferation and prognosis.Methods The expression of AQP8 in 127 patients'paraffin sections of tumors and adjacent tissues was detected by immunohistochemistry.All of the patients underwent TNBC radical surgery at the Fuxing Hospital of Capital Medical University from January 2010 to January 2013.The expression of vascular endothelial growth factor(VEGF)and Ki-67 in TNBC tissues were also detected by immunohistochemistry at the same time.The relationship between the expression of VEGF and Ki-67 in TNBC tissues and AQP8 were analyzed.Results AQP8 expression was significantly increased in TNBC tissues compared to adjacent tissues.This was accompanied by an abnormal expression of Ki-67 and angiogenesis-related marker VEGF.In addition,high expression of AQP8 was associated with invasive clinicopathological factors,decreased overall survival time(OS)and disease-free survival time(DFS).Multivariate analysis showed that AQP8 expression may be an independent predictor of decreased OS and DFS in TNBC patients.Conclusion The high expression of AQP8 is associated with angiogenesis,proliferation,and poor prognosis in patients with TNBC.