淫羊藿、黄芪、葛根有效组分复方对APPswe/PS1dE9双转基因AD模型小鼠海马CA3区hepcidin表达的影响

Effects of active components of Epimedium,Astragalus and Radix Puerariae on expression of hepcidin in hippocampus CA3 in APPswe/PS1d E9 double transgenic Alzheimer’s disease model mice

目的 探索黄芪、淫羊藿、葛根有效组分复方对APPswe/PS1dE9双转基因AD模型小鼠海马CA3区hepcidin表达的影响。方法 将30只10月龄的雄性APPswe/PS1dE9双转基因模型小鼠随机分为复方组、模型组和去铁斯诺(DFX)组,10只10月龄的雄性C57BL/6J小鼠作为正常对照组。用药结束后,取出小鼠的脑组织,应用免疫荧光、Real-time PCR和Western blot检测各组小鼠海马CA3区hepcidin的表达情况。结果 与正常对照组小鼠相比,模型组小鼠海马CA3区hepcidin的表达明显降低( P <0.05);淫羊藿、黄芪、葛根有效组分复方和DFX灌胃干预后,hepcidin表达水平较APPswe/PS1dE9双转基因AD模型小鼠海马CA3区明显升高( P <0.05)。复方组和DFX组相比,hepcidin表达水平差异无显著性( P >0.05)。结论 黄芪、淫羊藿、葛根有效组分复方可以上调hepcidin的表达,提示淫羊藿、黄芪、葛根有效组分复方在阿尔茨海默病的铁超载临床治疗方面具有重要的理论意义。

Aim To explore the effect of a combination of Astragalus, Epimedium and Pueraria on the expression of hepcidin in hippocampal CA3 region of APPswe/PS1 dE9 double transgenic AD model mice. Methods Thirty 10-month-old APPswe/PS1 dE9 double transgenic model mice were randomly divided into three groups: the compound group, the model group and the deferox(DFX) group, and ten 10-month-old C57 BL/6 J mice were as normal control group. Subsequently, the brain tissue of the mice was taken out, and the expression of hepcidin in the hippocampal CA3 region of each group of mice was detected by immunofluorescence combined with Real-time PCR and Western blot. Results Compared withnormal group, the mRNA expression of hepcidin was down-regulated inmodel group.Afterintragastric administrationof active components of Epimedium, Astragalus and Radix Puerariae and DFX, hepcidin expression levels increased significantly compared with those in APPswe/PS1 dE9 double transgenic AD model mice hippocampal CA3 area(P<0.05). Compared with DFX group, there was no significant difference in mRNA expression and hepcidin protein indicesin compound group(P>0.05).Conclusions The effective components of Astragalus, Epimedium and Radix Puerariae can up-regulate the expression of hepcidin, It is suggested that the effective components of Epimedium, Astragalus, and Radix Puerariae have important theoretical significance in the clinical treatment of Alzheimer?s disease with iron overload.