通腑饮对腹腔高压症大鼠血乳酸、肠黏膜通透性和近期预后的影响

Effects of Tongfu Drink on Short-term Prognosis, Intestinal Mucosal Permeability and Lactic Acid in the Blood of the Rats with Abdominal Hypertension

目的:探讨通腑饮对腹腔高压症大鼠的血乳酸、肠黏膜通透性和近期预后的影响及其相互关系。方法:将40只健康成年雄性SD大鼠随机分为A组和B组,每组20只。2组均通过向腹腔内注入氮气,建立腹腔高压症模型,2组均维持腹内压20 mm Hg,持续48小时,A组采用通腑饮方煎制成药液灌肠,每次5 mL,1次/6 h,共8次,B组同期采用等量生理盐水灌肠治疗。检测比较2组治疗前后的血乳酸水平,并统计2组大鼠48小时死亡率。48小时后采用EB和FITC-dextran方法检测2组小肠黏膜通透性。比较2组不同预后大鼠治疗前后的血乳酸水平和小肠黏膜通透性,分析腹腔高压症大鼠血乳酸水平和小肠黏膜通透性与48小时死亡率的关系。结果:与治疗前比较,A组治疗12小时、24小时及48小时的血乳酸水平均降低,B组治疗12、24及48小时的血乳酸水平则升高,且A组治疗12小时、24小时及48小时的血乳酸水平均低于B组(P<0.05)。A组48小时死亡率为10.00%(2/20),B组48小时死亡率为40.00%(8/20),A组48小时死亡率低于B组(P<0.05)。且2组存活大鼠治疗前后的血乳酸水平以及小肠黏膜通透性均低于其死亡大鼠(P<0.05)。Spearman分析结果显示,大鼠腹腔高压症血乳酸水平和小肠黏膜通透性与其48小时死亡率均密切相关。结论:通腑饮治疗腹腔高压症大鼠有助于降低其血乳酸、肠黏膜通透性并改善其预后,且大鼠血乳酸、肠黏膜通透性与近期预后均密切相关,可作为其近期预后评估的参考指标。

Objective: To discuss the effects of Tongfu drink on lactic acid, intestinal mucosal permeability and short-term prognosis of the rats with abdominal hypertension and their correlation. Methods: Forty healthy adult male SD rats were randomized into group A(n=20) and group B(n=20). The rats with abdominal hypertension were prepared by injecting nitrogen into abdomen in both groups, and maintaining intra-abdominal pressure at 20 mm Hg,for 48 hours continuously, group A accepted the enema of herbal decoction using Tongfu drink, 5 mL each time,once every six hours, altogether eight times, group B received the enema of equivalent physiological saline in the corresponding period. To detect and compare the levels of serum lactic acid before and after treating between both groups, and count mortality rate within 48 hours in both groups. EB and FZTC-dextran method were used to measure intestinal mucosal permeability in 48 hours. To compare the levels of serum lactic acid and intestinal mucosal permeability before and after treating of the rats in both groups, and analyze the correlation between the levels of serum lactic acid and intestinal mucosal permeability of the rats with abdominal hypertension and mortality rate within 48 hours. Results: Compared with before treating, the levels of lactic acid of group A reduced in 12, 24 and 48 hours after treating, while the levels of lactic acid of group B raised in 12, 24 and 48 hours after treating, and the levels of serum lactic acid of group A in 12, 24 and 48 hours after treating were lower than these of group B(P<0.05).48-hour mortality of group A was 10.00%(2/20), lower than 40.00% of group B(8/20)(P<0.05). The levels of lactic acid and intestinal mucosal permeability of survival rats in both groups were lower than these of the dead(P<0.05).The results of Spearman analysis showed that the levels of serum lactic acid and intestinal mucosal permeability were closely related to 48-hour mortality. Conclusion: Tongfu drink in the treatment for the rats with abdominal hypertension is helpful to lower the levels of lactic acid and intestinal mucosal permeability, and improve the prognosis, the levels of lactic acid and intestinal mucosal permeability are closely related to short-term prognosis,which could be taken as the reference indexes for the evaluation of short-term prognosis.