Beclin1、LC3和mTOR在食管腺癌中的表达及相关性研究

The Expression and Correlation of Beclin1, LC3 and mTOR in Esophageal Adenocarcinoma

目的探讨食管腺癌发生发展过程中自噬特点。方法采用RT-PCR和Western blot技术检测自噬相关蛋白Beclin1、微管相关蛋白L轻链3(LC3)和雷帕霉素靶蛋白(mTOR)在食管正常黏膜(normal esophagus,NM)、反流性食管炎(reflux esophagitis,RE)、Barrett食管(Barrett esophagus,BE)及食管腺癌(esophageal adenocarcinoma,EA)中的表达及意义,并对其与临床病理学的关系进行分析。结果自噬调控因子Beclin1、LC3在NM、RE和BE的表达阳性率均高于EA中的阳性率(P均<0.05),mTOR在RE和BE的阳性率均低于EA中的阳性率(P均<0.05),且Beclin1、LC3 在EA中的表达与肿瘤分化程度和TNM 分期有关,与年龄、性别无关;在RE、BE和EA组织中,Beclin1与LC3两者的表达呈正相关(r=0.393、0.346、0.413,P均<0.05),mTOR与LC3的表达呈正相关(r=0.259、0.254、0.023, P均<0.05)。结论联合检测Beclin1、LC3 及mTOR在食管腺癌中的表达有助于评估进展程度。

Objective To investigate the autophagy characteristics during the development of esophageal adenocarcinoma. Methods Real-time PCR and Western blot were employed to detect the expression of Beclin1, LC3 and mTOR in normal esophagus(NM), reflux esophagitis(RE), Barrett esophagus(BE), esophageal adenocarcinoma(EA). The relationship between them and clinicopathology was analyzed. Results The positive expression rates of Beclin1 and LC3 in NM, RE and BE were higher than those in EA (P<0.05). The positive expression rate of mTOR in NM, RE and BE were lower than that in EA(P<0.05). The overexpressions of Beclin1 and LC3 were related to differentiation degree and TNM degree, and were not associated with age and sex.In NM, RE, BE and EA, the expression of Beclin1 was positively correlated with LC3(r=0.393, r=0.346, r=0.413, P all<0.05) and the expression of LC3 was positively correlated with mTOR(r=0.259,r=0.254,r=0.023, P all<0.05). Conclusion The aberrant expression of Beclin1, LC3 and mTOR may be associated with the development and progression of esophageal adenocarcinoma.The simultaneous detection of Beclin1,LC3 and mTOR genes in EA may be helpful for the evaluation of the progressive degree.