Slc26a9基因在胃癌发生发展中的作用研究

The role of Slc26a9 gene in the genesis and development of gastric cancer

目的:研究Slc26a9基因在胃癌发生发展中的作用。方法:从胃癌手术患者的新鲜手术标本中选择200例配对组织,另取200例标本用于实时PCR与West Blot验证,分析Slc26a9基因在胃癌中的表达意义、SNP位点基因型分布频率和临床指标。结果:Slc26a9基因在胃癌组织中呈现出染色包浆阳性细胞,呈现出片状或者弥漫分布的状况,阳性率为56.5%。患者的Slc26a9基因SNP位点呈现出了3种不同的基因型。分布差异有统计学意义(χ^2=6.340,P=0.042)。结论:Slc26a9基因在胃癌发生发展中有显著上调的作用,病理组织检测得出,该基因组织负责转运的几种氨基酸水平,在患者体内均明显升高,表明此种基因会影响到患者细胞增殖,参与到肿瘤发生发展机制中,产生促进癌变作用。

Objective:To study the role of Slc26a9 gene in the genesis and development of gastric cancer.Methods:Paired tissues were selected from 200 fresh surgical specimens of gastric cancer patients in our hospital.Another 200 specimens were used for real-time PCR and WB validation.The significance of Slc26a9 gene expression in gastric cancer,the frequency of SNP genotype distribution and clinical indicators were analyzed.Results:Slc26a9 gene showed positive staining-coated cells in gastric cancer tissues,showing flaky or diffuse distribution,with a positiver ate of 56.5%.The SNP locus of Slc26a9 gene in the patient showed three different genotypes.There were significantly statistical differences in distribution(χ^2=6.340,P=0.042).Conclusion:Slc26a9 gene has a significant up-regulation in the development of gastric cancer.Pathological examination showed that the levels of several amino acids transported by Slc26a9 gene were significantly increased in patients,suggesting that Slc26a9 gene can affect the proliferation of patients cells,participate in the mechanism of tumorigenesis and development,and promote carcinogenesis.