数据挖掘BLQ复方中药对肝癌荷瘤小鼠的抑瘤作用及初步机制研究

Data mining of the antitumor effect of BLQ Herbal Medicine on hepatoma H22 tumor bearing mice and its possible mechanisms

目的:观察数据挖掘BLQ复方中药对肝癌荷瘤小鼠的抑瘤作用,并探讨其初步机制。方法:ICR小鼠适应性饲养后,建立H22荷瘤小鼠模型,并随机分为模型组、5-FU组、BLQ复方中药高、中、低剂量组,造模后第2日开始给药,模型组给予生理盐水灌胃,BLQ复方中药高、中、低剂量组给予同体积中药水煎剂灌胃,灌胃体积为0.1mL/10g,连续灌胃14d后处死小鼠,完整剥离肿瘤组织,称重,计算抑瘤率;取脾脏和胸腺,计算脾脏和胸腺指数;取血离心后用酶联免疫吸附法检测血清中白细胞介素-1(IL-1)、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ)含量变化。免疫印迹法(Westernblot)检测瘤组织Bax、Bcl-2、Caspase-3、Caspase-9蛋白表达。结果:与模型组比较,BLQ复方中药高、中、低剂量组抑瘤率分别为45.61%、47.72%及27.31%(P<0.01,P<0.05)。与模型组比较,BLQ复方中药高、中剂量组均能显著上调Bax、Caspase-3、Caspase-9蛋白表达(P<0.01),高剂量组则显著下调Bcl-2蛋白表达(P<0.01);低剂量组能显著上调Bax蛋白表达(P<0.01)。结论:BLQ复方中药能抑制肝癌小鼠肿瘤生长,可能是通过对细胞凋亡相关蛋白Bcl-2、Bax及Capase-3、Capase-9的调节实现的,是BLQ复方中药抑制肿瘤细胞生长的机制之一。

Objective: To investigate the antitumor effect of Data Mining BLQ Herbal Medicine on hepatoma H22 tumor bearing mice and its possible mechanisms. Methods: 50 ICR mice were randomly divided into five groups, model group, 5-FU group, BLQ high dose, medium dose and low dose groups. All the groups were subcutaneously injected with suspension of H22 hepatocellular carcinoma cells (2×10^7/mL) into the right anterior armpit. On the 2nd day after the modeling, the model group were given NS ig, and BLQ high dose group, medium dose and low dose groups were given the same volume of decoction, ig, with the dosage of 0.1ml/10g.After 14 days, all mice were sacrificed.Their tumor lumps were collected to weigh and calculate the inhibition rate and make pathological sections. Spleen and thymus were collected to calculate the spleen index and thymus index, enzyme-linked immunosorbent assay (ELISA) was used to examine interleukin-1(IL-1), tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) content in peripheral blood. Western blot method was adopted to detect the expression of Bax, Bcl-2, Caspase-3 and Caspase-9 protein expression. Results: Compared with model group, the inhibition rate of BLQ high dose, medium dose and low dose groups was 45.61%, 47.72% and 27.31% respectively. The expression of Bax, Caspase-3 and Caspase-9 protein were significantly up-regulated in BLQ high dose and medium dose groups. The expression of Bcl- 2 protein was down-regulate in BLQ high dose group as well as the expression of Bax protein was up-regulate in BLQ high dose group. Conclusions: BLQ herbal medicine could inhibit the growth of liver cancer in mice tumor blocks. It may be through the regulation of apoptosis-related proteins Bcl-2, Bax, Capase-3, and Capase-9.