长链非编码RNA lncAdam-9表达与早产胎膜早破关系的研究

Associating the expression of long-chain non-coding RNA lncAdam9 to premature rupture of fetal membranes

目的评估I型血小板结合蛋白基序的解聚蛋白样金属蛋白酶(Adamts)基因及其lncRNA在早产及胎膜早破中的表达状态.方法选取2017年3月至2018年3月在内蒙古自治区妇幼保健院产科分娩的孕妇共120例,根据孕妇的分娩孕周以及胎膜破裂与否,将孕妇分为早产胎膜早破组、早产非胎膜早破组、足月胎膜早破组和足月非胎膜早破组,每组标本各30例,在孕妇胎盘胎膜娩出后收集其破口边缘的胎膜组织,置于-80℃冰箱冻存.采用实时定量PCR方法,分别从4个组别各30例胎膜样本中分析Adamts-9及lncAdamts-9的相对含量.结果 Adamts-9及lncAdamts-9的相对含量在4个组中水平高低趋势接近一致,两者在胎膜早破组中的含量均要高于非胎膜早破组,且在足月胎膜早破组中的水平要高于其他组,差异均有统计学意义.结论 Adamts-9及lncAdamts-9可能参与了胎膜早破的内在调控机制.

Objective This study aims to evaluate how the expression of the adensin-like metalloproteinase ( Adamts ) gene and its lncRNA in type I platelet-binding protein motif affect preterm delivery and premature rupture of membranes.Methods From March 2017 to March 2018,a total of 120 pregnant women were recruited from the obstetrics and gynaecology department of the Inner Mongolian Maternal and Child Care Hospital.According to the gestational weeks of the pregnant women and conditions of their fetal membranes,the pregnant women were divided into four groups,namely premature delivery (PD) and premature rupture of membrane (PRM),PD and non-premature rupture of membrane (non-PRM),full-term delivery (FTD) and PRM,and FTD and non-RM.30 clinical cases were involved in each group.After delivery,a rupture at the margin of fetal membrane were extracted from the maternal placenta.The samples were refrigerated at -80℃.Real-time quantitative PCR was used to analyze relative expression of Adamts-9 and lncAdamts-9 of each group.Results The relative expressions of Adamts-9 and lncAdamts-9 were varied in a similar pattern.The expression was higher in PRM groups than the non-PRM groups;and the highest expression level was found in the group of FTD and PRM.The differences were statistically significant.Conclusion Both Adamts-9 and lncAdamts-9 are likely to play a role in the intrinsic regulation mechanisms of premature rupture of membranes.