基于label-free技术的梅花鹿(Cervus nippon)茸角蛋白组分比较

Comparative Proteomic Analysis of Sika Deer Antler Based on Label-free

旨在探究不同生长时期梅花鹿鹿茸的蛋白质组分信息,为鹿茸有效活性物质的挖掘提供理论依据。本研究利用label-free蛋白质组学技术及生物信息学方法对不同生长时期(10、20、40、60、130与360天)梅花鹿茸角蛋白质组分进行比较研究。结果显示,梅花鹿茸角含有丰富的蛋白质,10、20、40、60、130与360天蛋白质含量依次为65.35、70.90、74.00、82.25、56.00、28.02mg·g^-1。应用label-free蛋白质组学技术共鉴定出636种梅花鹿茸角蛋白质,其中218种蛋白质为显著差异表达,主要参与了蛋白质合成、发育、细胞骨架、转运等生物学过程。不同生长阶段茸角的蛋白质表达有各自的特点,为梅花鹿茸角药理活性成分的筛选及茸角相关产品的开发奠定理论基础。

The purpose of this study was to explore the protein component information of sika deer antler in different growth stages,and provide a theoretical basis for the exploration of effective active substances in antler.In this study,the proteome characteristic and alterations at 6 time points(10,20,40,60,130 and 360 d after casting)throughout antler growth cycle were analyzed based on label-free and bioinformatics methods.The result showed that sika deer antler was rich in protein,and the protein content in antler on 10,20,40,60,130 and 360 d were 65.35,70.90,74.00,82.25,56.00,28.02 mg·g^-1,respectively.A total of 636 proteins were successfully identified based on label-free,of which 218 proteins involved in protein synthesis,developmental process,cytoskeleton,transport were found to be significantly differentially expressed in different growth stages of antler.The protein expression in velvet horns in different growth stages has their own characteristics,which lays a theoretical foundation for the screening of pharmacological active components in sika antler horn and the development of velvet-related products.