一种螯合型Fe2+探针的构筑及性质

Construction and Fe2+ Sensing of a Chelation Based Fe2+ Probe

铁是人体的必需过渡金属元素,体内铁离子平衡的紊乱与多种疾病相关。发展铁离子探针,实现细胞和生命体中铁离子时空分布的跟踪与成像,对铁离子生理功能的研究具有重要价值。我们将BODIPY荧光团与三联吡啶通过乙烯基偶联,构建了新型Fe2+螯合型探针BTPY,通过比色法与荧光法结合,实现了Fe2+的特异性检测。探针最大激发波长582nm在可见光区,荧光发射波长678nm在近红外区,满足活体近红外成像的初步要求。BTPY与Fe2+/Fe3+结合导致吸收波长由573nm红移至607nm,溶液颜色由红色到蓝色。其他金属离子的结合不发生明显吸收峰和颜色变化。同时,Fe2+引起BTPY荧光猝灭而Fe3+对BTPY荧光不造成明显影响,实现了Fe2+与Fe3+的区分。

Iron is the most abundant essential transition metal in human body, and iron disorder has been reported to be associated with many diseases. Therefore, the development of fluorescent probes for Fe2+/Fe3+ imaging and offering spatial-temporal information of Fe2+/Fe3+ is of great significance. We chose BODIPY with good stability and high quantum yield as the fluorophore, and the terpyridine with high binding ability to Fe2+ as a chelating group. The probe BTPY for Fe2+ was constructed by integrating BODIPY with terpyridine at α-position by a vinyl group to form a large conjugated system. The NIR emission at 678 nm and the visible excitation at 582 nm suggest that this probe has the potential for in vivo imaging in animal model. BTPY was able to selectively bind Fe2+/Fe3+ with a bathochromic absorption shift from 573 to 607 nm and the solution color changed from red to pale blue. In addition, Fe2+ caused BTPY fluorescence quenching while Fe3+ did not significantly affect the fluorescence. Combining with the spectra change of absorption and emission, this probe can be used for visual detection of iron ions and is able to distinguish Fe2+ from Fe3+.