地塞米松对儿童自身免疫病患者外周血淋巴细胞早期凋亡的影响

Effect of dexamethason on early apoptotic lymphocytes in the peripheral blood of children with autoimmune diseases

目的 为了观察儿童自身免疫病患者 (以SLE为代表 )在应用地塞米松 2 4h前后细胞凋亡情况 ,对其外周血淋巴细胞早期凋亡率进行了检测。方法 应用最新的AnnexinV检测试剂盒及流式细胞仪检测早期凋亡细胞。结果 儿童SLE初发活动期患者外周血淋巴细胞早期凋亡率〔(2 .16 6± 1.345 ) %〕 ,明显低于经静脉注射地塞米松2 4h后的患儿〔(10 .4 96± 3.830 ) %〕及同年龄段正常儿童〔(12 .2 95± 4 .4 78) %〕 ;而后两者相比则无明显差异。结论儿童SLE活动期患者经过静脉注射地塞米松以后 ,外周血淋巴细胞凋亡率明显升高。其可能的机制为固醇类激素通过激活促进凋亡基因 ,从而促进大量淋巴细胞凋亡 ,使自身免疫病的发生受到控制。

Objective To determine the change of the apoptosis in children with autoimmune disease (such as SLE) after injecting dexamethason 24h by examining the early apoptotic lymphocytes in their peripheral blood.Methods Early apoptotic cells were made saliently by Annexin V FITC staining method and detected with flow cytometry.Results The percentage of the early apoptotic lymphocytes in the peripheral blood of active child SLE patients was significantly lower(2.166±1.345)% than that of the patients after injecting dexamethason 24h(10.496± 3.830)% and that of the normal healthy children (12.295±4.478)%.Besides,no difference was found between the patients after injecting dexamethason and the normal healthy donors.Conclusion The early apoptotic lymphocytes increase significantly in the peripheral blood of child SLE patients after injecting dexamethason 24h,that might stimulate the promotor gene to control the apoptosis of lymphocytes,then the autoimmune disease is controled.