双龙丸对过氧化氢所致内皮细胞胞浆钙离子浓度变化的调节作用

THE REGULATION EFFECT OF SHUANGLONGWAN ON [Ca^(2+)]I OF CULTURED ENDOTHELIAL CELLS MADE BY H_2O_2

本文建立了应用荧光指示剂Fura-2/AM测定培养的牛主动脉内皮细胞胞浆钙离子浓度([Ca2+]i)的方法,分别测定在正常状态下、单独加入过氧化氢(H2O2)时及同时加入H2O2和双龙丸药物血清(阳性与阴性对照组分别为心痛定及生理盐水的药物血清)时培养的牛主动脉内皮细胞胞浆钙离子浓度([Ca2+]i),探讨H2O2作用下[Ca2+]i的变化及双龙丸对这一变化的调节作用。结果:测得正常对照组内皮细胞的[Ca2+]i为96.58±14.20nmol/L,加入H2O2(0.05mmol/L)可使其[Ca2+]i增加到294.41±42.73nmol/L;双龙丸能部分拮抗H2O2升高[Ca2+]i,其高剂量组作用强于低剂量组,但均不及心痛定。实验结果证实了双龙丸能调节Ca2+代谢,具有一定的钙拮抗作用。

In order to study the regulation effect of Shuanglongwanon[Ca 2+ ]i of en-dothelial cells madeby H 2 O 2 .The methodfor determining[Ca 2+ ]i in cultured bovine aorta endothelial cells by using fluorescent indicator Fura-2/AMwas established and the ex-perimentwas carried on with7groups,they were normal cells,cells+blood serum of rats treated by normalsaline(normalcontrol),cells+H 2 O 2 ,cells+H 2 O 2 +bloodserumof rats treated by Shuanglongwanwith two dosage,cells+H 2 O 2 +blood serumof rats treated by nifedipine(positive control)and cells+H 2 O 2 +blood serumof rats treated by normal saline(negative control).Results:The[Ca 2+ ]i of normalcells was96.58±14.20nmol/L.Addedon0.05mmol/LH 2 O 2 ,theI of cells increased to294.41±42.73nmol/L.Shuang-longwanhas someantagonismon the increasing of the[Ca 2+ ]i madeby H2O2and the effect of high dose was more than that of low dose,but less than that of nifedipine.The ex-perimentsproved that Shuanglongwancan regulate Ca 2+ metabolismand has somecalciuman-tagonism.