海嘧啶注射剂对SGC-7901人胃癌细胞凋亡的实验研究

Study of Sea Pyrimidine on apoptosis of human gastric cancer cell SGC-7901

目的:揭示海嘧啶的抗肿瘤作用机理。方法:采用流式细胞仪测定海嘧啶对人胃癌细胞凋亡及细胞周期的影响;采用激光扫描共聚焦技术观测海嘧啶对人胃癌细胞内[Ca2+]i的影响及[Ca2+]i变化时Ca2+的来源。结果:海嘧啶可诱导肿瘤细胞凋亡,升高肿瘤细胞内[Ca2+]i浓度,[Ca2+]i升高来源于细胞外钙内流和细胞内钙释放。结论:海嘧啶的抗肿瘤作用机理为诱导肿瘤细胞凋亡,通过开放细胞膜钙通道和引起细胞内钙释放两条途径升高肿瘤细胞内[Ca2+]i,启动细胞凋亡机制,从而诱导肿瘤细胞凋亡。

Objective To uncover the anti-tumor mechanism of Sea Pyrimidine(SP), a compound Chinese-Western medicinal preparation containing 5-fluorouracil, extracts of Astragalus membranaceus(Fisch. ) Bunge, Sophora flavescens Ait. And certain sea weed. Methods Effect of SP on SGC-7901 gastric cancer cell cycle and apoptosis were determined by FCM and the source of Ca2+ during intracellular [Ca2+]; change, observed by laser scanning confo-cal microscopy. Results Sea Pyrimidine can induce apoptosis of SGC-7901 tumor cell and elevate its [Ca2+]i level. The source of Ca2+ was originated from extra-cellular [Ca2+]i and intracellular Ca2+ release. Conclusion The anti-tumor mechanism of SP is its action to induce apoptosis of tumor cell through opening of calcium channel and release of intracellular calcium to initiate cellular degradation resulting in tumor cell apoptosis.