发酵奶对二甲基肼诱发小鼠大肠隐窝上皮细胞微核和凋谢的影响

INHIBITORY EFFECT OF FERMENTED MILK ON DMH-INDUCED MICRONUCLEI AND APOPTOSIS IN THE COLON CRYPT EPITH ELIAL CELLS OF MICE

近交系C_(57)BL小鼠分别饮用发酵奶、牛奶和自来水。7天后给各组小鼠腹腔注射二甲基肼。24h后处死动物,取大肠固定,按卷帘法石蜡包埋,切片油镜观察20个纵切完整的隐窝,计数上皮细胞中出现微核和凋谢率。经统计学处理,发酵奶组的微核和凋谢率明显低于饮水和饮牛奶组。结果提示Shahani菌株发酵奶能明显抑制二甲基肼对小鼠大肠隐窝上皮细胞的诱变作用。

The effect of fermented milk on micronuclei and apoptosis induced by Dime-thylhydrazine (DMH) in the colon crypt cells of the mouse was studied. Fermented milk, milk and tap water were fed to three groups of C57BL mice. The animals were given DMH i.p. at dosage of 20mg/Kg body weight on the 7th day. The animals were sacrificed 24 h after infection. It was noted that fermented milk significantly inhibited DMH-induced micronuclei and apoptosis in the colon crypt cells. The experiment further examplified that the nuclear anomalies of th crypt cells in mice would be effectively used as a detective measure of colon tumorigenesis blockers