小剂量氚水诱发小鼠精原细胞染色体易位的研究

GENETIC EFFECT OF LOW DOSE TRITIATED WATER IN SPERMATOGONIA OF MALEMICE

本实验采用一次腹腔注射法中毒氚水。氚水注入量分别为3.22,6.44,11.06,21.76和31.23×10~2KBq/g.b.w。小鼠睾丸组织65天累积吸收剂量分别为0.070,0.140,0.242,0.476和0.684 Gy。中毒后65天处死动物。通过观察初级精母细胞D-MI中的多价体探讨氚水诱发精原细胞染色体易位的剂量效应关系。实验结果表明,易位率随氚水中毒剂量的增加而升高,其剂量效应关系可拟合为直线方程Y=0.581+0.0366X。精原细胞易位的产物主要为D-MI中的链状四价体,其占总畸变的77.8％;其次为链状六价体和锈状三价体+单价体,分别占总畸变的11.1％和9.7％;环状四价体最少,仅占1.4％。

Reciprocal translocation induced in spermatogonia were studied by cytological technique in the derived spermatocytes. Five groups of male mice were injected with tritiated water at active concentration of 3.22, 6.44, 11.06, 21.78 and 31.23 ×102KBq/gbw respectively. The corresponding accumulative doses in mouse testis tissue were 0.070, 0.140, 0.242, 0.470 and 0.684Gy respectively within the following 65 days,when the mice were kilied.The results suggested that the translocation frequencies increased with HTO doses.The dose response relation ship fitted well with the linear equation: Y= 0. 581 +0. 0366X. The shapes of the translocation configuration observed at D-MI were mainly chains of four (C-IV), Which reached 77.8% of the total translocations. The numher of chains of six (C-VI) or trivalent plus uaivalent (C-Ⅲ +Ⅰ) ranked second, which were 11.0% and 9.7% of the total repectively. Only a few, 1.4% of the total, ring of four were seen.