鼻咽假复层纤毛柱状上皮核的形态定量学研究

MORPHOMETRIC STUDY ON THE NUCLEI OF PSEUDOSTRATIFIED COLUMNAR EPITHELIUM IN NASOPHARYNX

作者采用图象分析仪检测88例鼻咽小淋巴细胞及其中21例鼻咽假复层纤毛柱状上皮、8例异型增生柱状上皮胞核的多个形态定量学参数。经分析比较后认为:(1)假复层纤毛柱状上皮胞核的面积较小淋巴细胞胞核增大一倍左右,而核形状较偏离圆形;(2)假复层纤毛柱状上皮中、表层胞核与基层核比较,其核面积、核圆度增大,而核椭圆形状因子、核规则形状因子没有差异,即各层胞核形状基本一致,各层中大多数胞核(80％以上)的长径与基底膜垂直。上述形态定量学特征为研究发生于鼻咽假复层纤毛柱状上皮的癌前病变提供了正常客观指标。

Several morphometric parameters of cell nuclei of pseudostratified columnar epithelium and nuclei of small lymphocyte in 88 nasopharyngeal biopsies were measured by means of image analyser. By comparison, it was showed that (1) The mean nuclear area of pseudostratified columnar epithelium cell nuclei was two-fold larger than that of small lymphocytes. (2) The mean nuclear area and the mean nuclear roundness of nuclei situated in the intermediate and the superficial layer were both increased as compared to those situated in the basal layer, while concerning to the mean nuclear form ellipsoidity(Form Ell) and mean nuclear form regularity (Form Ar), there was no difference among these three layers. (3) Majority (>80%) of the long axis of the nuclei of columnar cells situated in the basal, the intermediate or the superficial layer, all were perpendicular to the epithelial basement membrane. The above findings would provide a basis for studying the precancerous lesions of nasopharyngeal epithelium.