硅酸盐细菌NBT菌株解钾机理初探

MECHANISM OF POTASSIUM RELEASE FROM FELDSPAR AFFECTED BY THE STRAIN NBT OF SILICATE BACTERIUM

摇瓶条件下 ,对硅酸盐细菌NBT菌株发酵液及其代谢产物分解钾长石的作用进行了研究。结果表明 ,培养 48h的NBT菌株发酵液能活化钾长石中的钾、硅、铝。 2 8℃振荡 1 0天后的滤液中K ,SiO2 ,Al2 O3含量分别达 1 84.1、39.8和 1 2 .6mgL- 1 ,分别比灭菌发酵液中的K ,SiO2 ,Al2 O3含量增加 1 0 6 .1 %、63 .1 %和 1 33 .3%。NBT菌株发酵液中含有大量的有机酸、氨基酸、荚膜多糖。摇瓶试验表明 ,三者都有较强的分解钾长石的能力 ,2 8℃振荡 1 0天后的滤液中的K含量分别达 1 1 0 .8、84.9和 1 9.7mgL- 1 。另外 ,三者间有明显的协同作用 ,三者的混合液可使解钾能力提高 62 .2 %。三者分解钾长石的能力是通过酸溶和络合作用来实现的。

The study on dissolution of potassium from feldspar powder by fermented broth and the metabolites of the strain NBT was carried out in shake flask. The results showed that K,Si,Al from feldspar was activated by fermented broth cultured for 48 hours. The contents of K,SiO 2,and Al 2O 3 in the fermented broth were 184.1,39.8 and 12.6 mg L -1 respectively after shaking with feldspar in it at 28℃ for 10 days and were 106.1%,63.1% and 133.3% respectively over that the control of sterilized fermented broth; In addition,a lot of organic,ammonia acids and capsular polysaccharide were detected in the fermented broth. The shake flask study showed that the metabolites had the potential of dissolving the feldspar and the contents of K in the solution were 110.8 mg L -1 for organic acids,84.9 mg L -1 for ammonia acids and 19.7 mg L -1 for capsular polysaccharide. The effect of K release was increased by 62.2% by the mixed metabolites. The dissolution of feldspar was through the acid dissolution action and chelation of the metabolites.