LAK细胞和rIL-2治疗小鼠脑胶质瘤及IL-7对LAK细胞活性的调节作用

STUDY OF IMMUNOTHERA Y OF CEREBROGLIOMA IN MICE BY USING LAK CELLS AND IL-2 AND REGULATION OF LAK CELLS BY IL-7

作者在应用LAK细胞和IL—2过继治疗颅内接种203—glioma脑胶质瘤的C_(57)BL/6小鼠的研究中,对LAK细胞和IL—2的输入剂量和途径等进行了系统分析。表明LAK细胞即可延长带瘤鼠的生存时间,但要求较高的LAK细胞浓度;颅内输入LAK细胞的疗效明显高于静脉输入;输入入IL—2能明显增加LAK细胞的疗效;颅内输入IL—2对LAK细胞疗效的增强作用明显优于静脉输入。在IF—7对LAK细胞活性调节作用的研究中,发现IF—7能诱导LAK细胞产生,并与IL—2有协同作用;IL—7诱导的LAK细胞活性不能被抗IL—2抗体所抑制。认为,脑胶质瘤术后颅内应用LAK细胞和IL—2是一种有前途的免疫疗法。IF—7有增强LAK细胞活性及降低IL—2副作用的功能。

In the present study, animals given injections of 5×10 5 203 - gliomacells alone or cultured spleen cells without IL-2 had a mean survival time of 3 weeks, co-mepared to 5.05 weeks (P<0.01) for mice treated with 2.5×10 6 LAK cells and 7.92week-(P<0.00l) for 107 LAK cells. We observed on adoptive immunotherapy of mouse glioma with LAK cells administrated either intracerebrally or iv. The results showed the mean survival time was prolongated by LAK cells administrated ic, but not iv. After 2 days that the mice were implaeted 203-glioma cells and LAK cells intracerebralty, various concentration of IL-2 were administrated ic or iv. It found that IL-2 enhanced killer activity of LAK cells in vivo and, intracerebral administrate of IL-2 was more effective than intravennous. IL-7 could induce the development of LAK cells in murine splenocyte cultures. The effect on induction of LAK cells by IL-7 was virtually independent of IL-2. LAK cell activity induced by IL-7 was not inhibited by an IL-2 neutralizing antiserum.