应用流式细胞术研究食管上皮细胞恶性转化的特性

ANALYSIS OF MALIGNANT TRANS FORMATION CHARACTERISTICS OF RAT ESOPHAGEAL EPITHELIUM USING FLOW CYTOMETRY

本文用先进的流式细胞技术(Flow Cytometry)研究了三种不同状态的大鼠食管上皮细胞:正常(NRE),不典型增生(DRE)和恶性转化的RE_(25-3),结果发现NRE和DRE的DNA指数(DI)相似为1.0,而RE_(25-3)则高达1.6—3.36,显示出明显的差异;细胞周期分析,S期细胞比例NRE为5.04％,DRE和RE_(25-3)均明显上升为29.92％和48.85％,差异十分明显。将RE_(25-3)接种至裸鼠,其S期细胞比例下降为12.24％,表现出宿主的影响。

The DNA content of the epithelium cells and the characteristics of cell proliferation cycle of various phase of malignant transformation were analysed by flow-cytometry. Comparison has been made amony three cell lines withdifferent proliferation capacityf33the normal esophgeal epithelium(NRE),the anaplasticepithelium induced by NSEE(DRE) and RE25 -3 (malignant transformation cell from DRE) Without using carcinogen in vitro .The experimental results showed that, NRE cells and DRE :ells have similar DNA content, DI-1.0, while the DI of various generations of the kE25-3 cells incerased to 1.60--3.36. The analysis of the cell proliferation cycle showed that the percentage of S phase cells was 5.04% in NRE, 29.92% in DRE, while in R-E5-3 it increased froml0.62%to 45.85% much higher than NRE and DRE. When RE25-3 was transplanted to the nude mice (RE25/N) its S phase cell decreased markedly which impress that the proliferation capacity of the cell may be affected by the immunity of the host.