肿瘤患者血中多胺的高效液相色谱分析

HPLC DETERMINATION OF POLYAMINES IN BLOOD OF TUMOR PATIENTS

本文报告肿瘤患者(81例),非肿瘤病人(36例)及正常成人(34例)血中多胺的测定结果,表明肿瘤患者血中的多胺总量高于正常人(P<0.01)及非肿瘤病人(P<0.01),精胺、精脒也有不同程度的增高。而非肿瘤病人与正常人血中多胺总量无明显差别(P>0.05)。提示血中多胺测定对癌症早期辅助诊断具有一定意义。高效液相色谱法实验重现性好,准确度高,采血量小,快速,是测定全血中痕量多胺的可行方法。

The amounts of polyamines blood of tumor patients ( 81 samples), non-tumor pationts (36 samples)and normal persons (34 samples ) were determined by H-PLC. The results indicated that the levels of total polyamines, spermine and spermidine in blood of the tumor patients were higher than those in bbod of normal subjects. Our studies suggested that determination of blood polyamines was valuable for early diagnosis of cancers. The HPLC method for the determiation of trace polyamies needs only very small blood sample and has been proved to be an accurate, reproducible and rapid moth-od.