杜仲优树的组培繁殖技术

Tissue Culture of Superior Eucommia Clone

以本校杜仲优树采穗圃的 2号优树 (代号黔 9)的新梢为试材进行组培繁殖 ,不同组合的培养基上试验得出 ,MS+ 6- BA1.0 + NAA0 .2 +蔗糖 4%对嫩梢诱导率最高 ,达 82 .5 % ;MS+6- BA1.0 + BAA1.0 对茎尖顶梢诱导率达 87.5 % ,MS+ IAA1+ BA1对幼梢的促长效果最佳。有效的生根培养基为 1 /2 MS+ GA0 .5+蔗糖 6% ,生根率 2 0 .7%。

Different explants from superior Eucommia clone No.2 were cultured to regenerate the mother plant rapidly by clone. The result are as follows: MS+6 BA 1.0 +NAA 0.2 +sucrose 4% was the optimum axillary sprout inducing medium, on which 82.5% axillary buds shot, apex sprout inducing medium: MS+6 BA 1.0 , on which 87.5% stem apex shot; plantlet growth medium: MS+IAA 1.0 +6 BA 1.0 ; rooting medium: 1/2MS+GA 0.5 +sucrose 6%, rooting rate arrived 20.7%.