抗鸡大肠杆菌F11菌毛单克隆抗体的研制

Preparation of Monoclonal Antibodies Specific for F11 Fimbriae of Pathogenic Chicken Exsherichia coli

将充分菌毛化的大肠杆菌C1976_79(pap +)经 4‰甲醛灭活作为免疫原免疫BALB/c小鼠 ,经淋巴细胞杂交瘤技术 ,用间接ELISA筛选获得FA1、FB11两株抗FB11菌毛单抗 ,其ELISA效价分别为 18log2 和 15log2 试管凝集价分别为 8log2 和 6log2 。免疫印迹实验表明 :两株单抗均能与鸡大肠杆菌F11菌毛反应 ,识别分子量为 18kD左右的菌毛蛋白。同时对猪病原性大肠杆菌进行检测 ,结果均无交叉反应。这表明F11菌毛是不同于K88、K99、987P、F4 1及F18等粘附素的一种大肠杆菌菌毛。

Escherichia coli C1976_79 posses F11 fimbriae,was inoculated BALB/c mice directly as immunogen to prepare specific monoclonal antibody.2strains of monoclonal antibody hybridoma cells were obtained by indirect Enzyme_linked Immunosrbent Assay(ELISA).All the 2 strains were specific to the F11 fimbriae through Western_blotting.When use the antibody as probe to check the fimbrias types with Escherichia coli.such as K88,K99,987P,F41 and F18.The result shows that the F11 fimbriae of pathogenic avian Escherichia coli are different with those adhesive factors.