摘要
CEF_9是传染性法氏囊病病毒超强毒株vvIBDV_Gx在鸡胚成纤维细胞上传代致弱过程中的第 9代毒。我们对其分子生物学及生物学特性进行了研究 ,结果表明其VP2基因序列既具有超强毒的特征 ,又兼有部分致弱毒的特征 ;其致病性介于超强毒株和致弱株之间 ;在体内外均不稳定的 ,体外继续传代可继续致弱 ,回归体内可迅速返强。这说明CEF_9是vvIBDV驯化时 ,毒力强弱转化的中间过渡形式。
CEF_9 is the ninth generation in the process which vvIBDV was attenuated through serial passaging in CEF. The results of researching on its molecular biology and biological characterizations shows that: the VP2 gene of CEF_9 has some characters of vvIBDV strain as well as some characters of attenuated strain, pathogenicity of CEF_9 is between vvIBDV strain and attenuated strain; and CEF_9 is not stable due to the fact that it could be attenuated in vitro and could rapidly atavistic in vivo. All the results demonstrate that CEF_9 is an intermediate form from high virulence to low virulence during the attenuation.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2002年第6期456-458,共3页
Chinese Journal of Preventive Veterinary Medicine
基金
欧盟合作项目 (ERBIC18CT980 330 )
