鸡肾型传染性支气管炎病毒组织嗜性的研究

Studies on the histotropism of avian nephropathogenic infectious bronchitis virus

本研究用鸡肾型传染性支气管炎病毒 (肾型 IBV) C90 0 1株人工感染 14日龄 SPF鸡 ,于接种后定期采集病鸡的各组织器官制备成石蜡切片 ,用建立的检测石蜡切片中肾型 IB病毒的免疫酶组化染色技术 ,对人工感染肾型传染性支气管炎 (肾型 IB)鸡发病后病毒的组织器官亲嗜性和动态分布规律进行了研究。结果表明 ,肾型 IBV在胞浆内复制 ,主要亲嗜气管黏膜的上皮细胞和固有层腺体细胞、肺各级支气管上皮细胞以及肺房和呼吸性毛细管上皮细胞、气囊上皮细胞、肾和输尿管上皮细胞、消化道上皮和固有层腺体细胞、肝小叶间胆管上皮细胞、胰腺导管上皮细胞和腺泡上皮细胞、法氏囊淋巴滤泡髓质区淋巴细胞和网状细胞、胸腺小叶髓质区淋巴细胞和网状细胞、脾小体淋巴细胞、盲肠扁桃体弥散性淋巴组织的淋巴细胞以及心肌细胞 ,病毒出现的先后顺序为气管、肺脏、肾脏、输尿管 ,消化道、法氏囊、肝脏、胰脏、胸腺和气囊 ,盲肠扁桃体 ,脾脏 ,心肌。病毒在肾脏和输尿管持续 2 0天 ,气管为 13天 ,消化道和法氏囊为 11天 ,肝脏、胰脏和肺为 10天 ,胸腺为 6天 ,气囊为 5天 ,盲肠扁桃体、脾脏。

In this study,fourteen day old specific pathogen free chickens were artificially infected with a local strain of nephropathogenic infectious bronchitis virus designated as C9001,paraffin embedded tissuse sections were prepared at 0.5,1,2,3,4,5,6,7,8,9,10,11,13,15,17,20,22,25days postinfection.The tissue sections were stained with an indirect immunoperoxidase technique based on hyperimmune serum and harris' staining method to detect avian nephropathogenic infectious bronchitis virus antigens,optimal dilution titers of the hyperimmune and goat anti mouse lgG conjugated with HRP were 1∶100 and 1∶400.Viral antigens were localizated in the cytoplasm of the epithelia of tracheal mucosa,glandulous epithelia of tracheal lamina propria,epithelia of bronchus of pulmo,epithelia of artium and respiratory capillary,epithelia of air sacus,epithelia of renal tubules and urter,epithelia of digestive tract and glands of lamina propria,epithelia of biliary ducts among hepatic lobules,epithelia of pancreatic ducts and pancreatic glands,lymphocytes and reticulocytes in medulla of bursal follicll and thymic lobules,lymphocytes of splenic corpuscle and cecal tonsil,and myocardium.The sequence that viral antigens appeared in the organs was trachea and lung,kindey and urter,digestive tract and bursa of Fabricius,liver,pancreas,thymus and air sacus,cecal tonsil,spleen,myocardium.Periods that viral antigens existed in the various organs were not egual,20 days in kindey and urter,13 days in trachea,11 days in the digestive tract and bursa of Fabricius,10 days in liver and pancreas and lung,6 days in thymus,5 days in air sacus,1 days in cecal tonsil,spleen and myocardium,respectively.