Quantitative Study of Basic Fibroblast Growth Factor in Vitreous with Proliferative Vitreoretinopathy

Objective:To quantitatively study basic fibroblast growth factor(bFGF)in the vitreous ofproliferative vitreoretionopathy(PVR)inorder to understand the role of bFGFin the develop-ment of PVR.Method:High sensitive sandwich enzyme immunoassay technique(ELISA)was used to measure bFGF level in vitreous of normal eyes,the eyes of PVR-Cor pVR-Dgrade,eyes of vitreous hemorrhage and the serum levels of bFGF in PVR-Dpatients.Results:The levels of bFGF in the vitreous were:median5.20ng/L,quartile15.47ng/Lin20normal eyes;median3.12ng/L,quartile10.48ng/Lin35PVR-Ceyes;median46.56ng/L,quartile113.96ng/Lin26pPVR-Deyes;median1.40ng/L,quar-tile6.25ng/Lin25vitreous hemorrhage eyes.The vitreous bFGF level in PVR-D group was significantly higher than that in the normal group,PVR-Cgroup and vitreous hemorrhage group(P<0.01).The mean of serum-bFGFlevel was18.33±3.39ng/L.The vitreous bFGFlevel ofPVR-Dgroup was significantly higher than serum-bFGFlev-el(P<0.01).And the vitreous-bFGFlevel in PVR-Dgroup was significantly higher in larger retinal tear subgroup.Conclusion:The results suggested that bFGF is involved in the development of PVR.Eye Science2000;16:7-10.

Objective: To quantitatively study basic fibroblast growth factor (bFGF) in the vitreous of proliferative vitreoretinopathy (PVR) in orderto understand the role of bFGF in the development of PVR. Method: High sensitive sandwich enzyme immunoassay technique(ELISA) was used to measure bFGF level in vitreous of normal eyes, the eyes of PVR-C or PVR-D grade, eyes of vitreous hemorrhage and the serum levels of bFGF in PVR-D patients. Results: The levels of bFGF in the vitreous were: median 5. 20ng/L, quartile 15. 47 ng/L in 20 normal eyes; median 3. 12ng/L, quartile 10. 48 ng/L in 35 PVR-C eyes; median 46. 56 ng/L, quartile 113. 96 ng/L in 26 PVR-D eyes; median 1. 40 ng/L, quartile 6. 25 ng/L in 25 vitreous hemorrhage eyes. The vitreous bFGF level in PVR-D group was significantly higher than that in the normal group, PVR-C group and vitreous hemorrhage group ( P < 0. 01). The mean of serum-bFGF level was 18. 33 ± 3. 39 ng/ L. The vitreous bFGF level of PVR-D group was significantly higher than serum-bFGF level ( P < 0. 01). And the vitreous-bFGF level in PVR-D group was significantly higher in larger retinal tear subgroup. Conclusion: The results suggested that bFGF is involved in the development of PVR. Eye Science 2000; 16: 7 - 10.