HLA class Ⅰ expression in primary hepatocellular carcinoma

AIM：To investigate whetherCTL vaccine therapy is suitable for primary hepatocellular carcinoma(HCC)from the viewpoint of HLA classIantigens expression.METHODS:The immunocytochemistry,image analysis,flow cytometry,and labeled streptavidin bioti(LSAB)method of immunohistochemistry were applied respectively to study 4HCCcell lines(e.g.Alexander,HepG2,SMMC-7721,andQGY-7703)cultured in vitro and 6frozen tissue specimens of HCC.RESULTS:The positive control cell line Raji had very strong positive staining,Most mitotic and nonmitotic cells of the 4HCCcell lines had various intensity of HLAclassⅠantigens expression.The negative control cell K562 and the control slides of all the cell lines had no positive staining,In the 6HCCspecimens immunohistochemically studied,histological normal hepatocytes had no or very weak positive staining and the liver sinus had very strong positive staining.Most HCCcells in the sections from the 6HCCspecimens had strong positive HLAclassⅠantigens staining.The positive staining was located in the cytoplasm,the perinuclear area,and at the cell membrane of the liver cancer cells.Flow cytometry also revealed that Raji and those 4HCCcell lines had strong HLAclassⅠantigens expression.which was confirmed quantitatively by the image analysis.It showed that the objective grayscale values of Raji and those 4HCCcell lines were significantly different from that of K562(Raji114.04±10.94,Alexander165.97±5.35,HepG2167.02±12.60,QGY-7703161.46±7.13,SMMC-7721 165.93±5.21,K562244.89±4.60,P<0.01).Significant differences were also found ebtween Raji and the 4HCCcell lines.CONCLUSION:HCC cells express HLA classI antigens strongly,Fromthis point of view.the active specific immunotherapy of CTL vaccine is suitable and practicable for HCC.

AIM:TO investigate whether CTL vaccine therapy is suitable for primary hepatocellular carcinoma(HCC) from the viewpoint of HLA class Ⅰ antigens expression. METHODS:The immunocytochemistry,image analysis, flow cytometry,and labeled streptavidin biotin(LSAB) method of immunohistochemistry were applied respectively to study 4 HCC call lines(e.g.Alexander, HepG2,SMMC-7721,and QGY-7703)cultured in vitro and 6 frozen tissue specimens of HCC. RESULTS:The positive control cell line Raji had very strong positive staining.Most mitotic and nonmitotic cells of the 4 HCC cell lines had various intensity of HLA class Ⅰ antigens expression.The negative control cell K562 and the control slides of all the cell lines had no positive staining.In the 6 HCC specimens immunohistochemically studied,histological normal hepatocytes had no or very weak positive staining and the liver sinus had very strong positive staining.Most HCC cells in the sections from the 6 HCC specimens had strong positive HLA class Ⅰ antigens staining.The positive staining was located in the cytoplasm,the perinuclear area,and at the call membrane of the liver cancer calls.Flow cytemetry also revealed that Raji and those 4 HCC cell lines had strong HLA class Ⅰ antigens expression,which was confirmed quantitatively by the image analysis.It showed that the objective grayscale values of Raji and those 4 HCC cell lines were significanUy different from that of K562(Raji 114.04± 10.94,Alexander 165.97±5.35,HepG2 167.02±12.60, QGY-7703 161.46±7.13,SMMC-7721 165.93±5.21, KS62 244.89±4.60,P<0.01).Significant differences were also found between Raji and the 4 HCC call lines. CONCLUSION:HCC cells express HLA class Ⅰ antigens strongly.From this point of view,the active specific immunetherapy of CTL vaccine is suitable and practicable for HCC.