摘要
AIM: To investigate whether the current manipulation ofliver resection for hepatocellular carcinoma(HCC)causesdissemination of liver tumor cells into blood circulation.METHODS: Fifty patients with HCC, but without evidencesof metastases, were enrolled for the study. Samples ofperipheral blood before skin incision and after abdominalwall suture, and those of hepatic venous blood and portalvenous blood after liver parenchyma dissection,wereobtainedAFP-mRNA was extracted from the blood samplesand detected with reverse transcription polymerase chainreaction assay. The changes of its expression during theoperation were accessed by the ratio of fluorescence of AFPand β-actin primers-amplified products.RESULTS: The rate of its expression before and afteroperation in peripheral blood, and during operation in portalvenous and hepatic venous blood was 42.0 %, 34.0 %, 42.0 %and 56.0 % respectively, There was no difference betweenthem. However, the ratio of fluorescence of AFP and β-actinprimers-amplified products from hepatic venous blood duringoperation is significantly higher than the other three typesof samples (P＜0.05).CONCLUSION: the current manipulation of liver resectionfor HCC can cause the releases of AFP mRNA-expressedcells from the liver, probably including tumor cells, intohepatic vein. These cells may spread into blood circulationand become the source of recurrence after operation.
AIM:To investigate whether the current manipulation of liver resection for hepatocellular carcinoma(HCC)causes dissemination of liver tumor cells into blood circulation. METHODS:Fifty patients with HCC,but without evidences of metastases,were enrolled for the study.Samples of peripheral blood before skin incision and after abdominal wall suture,and those of hepatic venous blood and portal venous blood after liver parenchyma dissection,were obtained.AFP-mRNA was extracted from the blood samples and detected with reverse transcription polymerase chain reaction assay.The changes of its expression during the operation were accessed by the ratio of fluorescence of AFP and β-actin primers-amplified products. RESULTS:The rate of its expression before and after operation,in peripheral blood,and during operation in portal venous and hepatic venous blood was 42.0 %,34.0 %,42.0 % and 56.0 % respectively,There was no difference between them.However,the ratio of fluorescence of AFP and β-actin primers-amplified products from hepatic venous blood during operation is significantly higher than the other three types of samples(P<0.05). CONCLUSION:the current manipulation of liver resection for HCC can cause the releases of AFP mRNA-expressed cells from the liver,probably including tumor cells,into hepatic vein.These cells may spread into blood circulation and become the source of recurrence after operation.
作者
Xiao-Ping Chen Hong Zhao Hepatic Surgery Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China Xi-Ping Zhao Department of Clinic Immunology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,Hubei Province,China
