乙型肝炎病毒BCP变异与慢性理症肝炎关系的探讨

RELATIONSHIP BETWEEN HBV BCP MUTANTS AND CHRONIC FULMINANT HEPATITIS B

目的 :探讨乙型肝炎病毒慢性感染中C基因启动子 (BCP)变异与慢性重症肝炎的关系。方法 :采用PCR微板核酸杂交结合ELISA检测显示技术 ,检测 74例乙型肝炎病毒慢性感染者BCP区核苷酸 (nt) 176 2碱基A→T和 176 4碱基G→A联合突变。结果 :在 74例乙型肝炎病毒慢性感染者中检出BCP区T176 2A突变 2 4例 (32 .4 % ) ,BCP变异在重症肝炎的发生率为 6 3.6 % (7/ 11)显著高于非重症肝炎的发生率 2 7% (17/ 6 3) (P<0 .0 5 )。结论 :BCP变异似可引起病情加重。

Objective:To investigate the mutant significance of HBV BCP in patients with chronic fulminant hepatitis B. Methods:Mutants (nt1?762A→T and 1?764G→A) of HBV BCP in 74 patients with chronic hepatitis B virus infection were studied by polymerase chain reaction combined with ELISA. Results:The T 1?762 and A 1?764 mutant in HBV BCP region were found in 24(24/74) patients. The positivity rates of mutant in chronic fulminant hepatitis B and in non-fulminant groups were 63.6%(7/11) and 27%(17/63) respectively ( P <0.05). Conclusion:The T1?762 A 1?764 mutants in HBV BCP region may be correlated to the occurrence of fulminant hepatitis.