摘要
目的 :了解胰岛素样生长因子1 (IGF1 )对体外生长的兔关节软骨细胞增殖以及细胞超微结构的影响。方法 :应用兔关节软骨细胞体外单层培养 ,对照组培养液为含 1 0 %小牛血清DMEM ,实验组在培养液DMEM中加入IGFl使其终浓度为 1 0 0 μg·L- 1 ,作用细胞 1周 ,得出细胞生长曲线 ,并分别测得两组DNA含量。同时观察IGFl 作用后 ,软骨细胞超微结构的变化。结果 :结果显示IGFl能明显促进培养软骨细胞增殖。实验组DNA含量 ,与对照组相比 ,有统计学意义 (P<0 .0 1 )。在电镜下 ,细胞主要表现为增殖性改变 ,说明细胞代谢活跃。结论 :IGFl 可刺激软骨细胞增殖 。
Objective: To study the effects of insulin like growth factor 1(IGF 1) on proliferation and ultrastructure of articular chondrocytes.Methods:The monolayer cells obtained from 4-week rabbits were cultured in DMEM containing 10% fetal calf serum and were treated with IGF 1 at 100μg·L -1 dose for certain period.DNA content and cell number were examined.Then the change of cell ultrastructure before and after IGF 1 treatment was observed.Results:The authors found that IGF l stimulated cell proliferation,in the group treated with IGF l was significantly higher than control( P <0.01).Meantime the proliferation image of the cell could be observed by electron microscopy.Conclusion:It was showed that cell proliferation in the cultured articular chondrocytes were stimulated by IGF 1.
出处
《中国矫形外科杂志》
CAS
CSCD
2002年第12期1205-1207,共3页
Orthopedic Journal of China
关键词
关节软骨细胞
胰岛素样生长因子
细胞培养
超微结构
Articular chondrocytes
Insulin like growth factor 1(IGF l)
Cell Culture
Utrastructure