川芎嗪预处理对心肌缺血再灌注所致血管内皮细胞损伤的保护作用

Ligustrazini Preconditioning Protects Ehdothelium From Myocardial Ischemia and R eperfussion Injury in Rat

目的 :用心肌缺血预处理的整体动物模型 ,观察川芎嗪预处理对麻醉大鼠心肌缺血再灌注所致血管内皮细胞损伤的保护作用。方法 :动物麻醉后 ,在人工呼吸状态下 ,开胸 ,结扎左冠状动脉。单纯缺血再灌注组 :结扎冠脉 3 0min ,其后再灌 12 0min ;缺血预处理组 :冠脉结扎5min ,再灌 5min ;然后 ,再次结扎冠脉 3 0min ,其后再灌 12 0min ;药物预处理组 :川芎嗪 2 0mg·kg-1 连续静脉给药 5min ;5min后 ,结扎冠脉3 0min ,其后再灌 12 0min ;假手术组 :冠脉下穿线 ,不做任何处理。实验结束后 ,腹主动脉采血 ,分离血浆。用放免法 (RIA)测ET ,TXB2 /6 酮 PGF1α,降钙素相关基因肽 (CGRP)。结果 :川芎嗪预处理降低缺血再灌后ET及TXB2 的释放 ,而 6 酮 PGF1α的释放增加 ,对CGRP没有影响。结论

In order to understand whether ligustrazini precondit ioning protects ehdothelium from myocardial ischemia and reperfussion injury. Method: The rats were randomly assigned to four groups.Group 1 r ats received a 30 min coronary occlusion followed by 120 min reperfusion.Gropu 2 rats were preconditioned with 5 min coronary occlusion followed by 5 min reperf usion before the 30 min coronary occlusion/120 min reperfusion.Group 3 rats rece i ved ligustrazini:20 mg·kg -1 before the 30 min coronary occlusion/120 min reperfusion.Group 4 rats,control group were subjected to no treatment.The level of ET,TXB 2/6 keto PGF 1α ,and CGRP was determined by RIA.Results: Ligustrazini preconditioning increased the level of 6 keto PGF 1α with raduced release of ET and TXB 2.No change of CGRP conce ntration was found.Conclusion: The results indicated that ligustrazini precondition ing may protects endothelium from myocardial ischemia and reperfussion injury.