湖北地区汉族健康人群白细胞介素-1α基因启动子-889C/T位点多态性的研究

Study of The Polymorphism at The Position -889C/T in The Promoter of Interleukin-1 Alpha

目的 :了解湖北地区汉族健康人群白细胞介素 1α基因 (IL 1A )启动子 -889位点的单核苷酸多态性 (SNP) ,比较其在不同种族间的分布特点。方法 :采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析的方法 ,检测 184名健康者IL 1A启动子 -889位点的SNP ,分析其基因型和等位基因频率。结果 :CC、CT、TT三种基因型 ,以C等位基因发生频率最高 ( 92 .4% ) ,T等位基因次之 ( 7.6% ) ;与挪威人群比较 ,其基因型和等位基因频率均存在极显著性差异 (P <0 .0 0 1) ,而与日本人群相比也有显著差异 (P <0 .0 5)。结论 :IL 1A启动子 -889位点存在SNP ,其在不同种族间的分布存在明显的差异 。

To observe the single nucleotide polymorphism (SNP) i n the promoter of interleukin 1 alpha at position -889 in the healthy Chinese H an Population of Hubei,and to compare and analyze the distributions of single nu cleotide polymorphism among different races.Method: Using PCR RFLP(NcoⅠ) method,we detected the IL 1A(-88 9) polymorphism in 184 healthy people,then calculated and analyzed the genotype and allele frequencies.Results: We found three genotypes,CC,CT and TT.The frequency of allele C (92.4%)is the highest,then follows the allele T(7.6%).For the genot ype and allele frequencies,significant difference between Norwegian and Chinese was observed ( P<0.001),but weak difference between Japanese and Chinese ( P <0.05).Conclusion: There is a SNP in the promoter of IL 1A(-889),whose distribution is significant different among different races.The difference will probably affect the incidence and symptom of some diseases.