肿瘤坏死因子基因多态性与乙型肝炎的相关性研究

Genetic Polymorphisms of TNF and Susceptibility of HBV Infection

目的 :探讨肿瘤坏死因子 (TNF)基因多态性与乙型肝炎易感性的关系。方法 :以病例 对照的研究方法 ,采用PCR RFLP技术检测 14 6例乙型肝炎患者和 165名正常对照者TNF α和TNF β基因型。 结果 :TNF αG/G和G/A基因型在病例组各占 95%和 5% ,对照组中分别为 88%和 12 % ,二组比较 ,基因型和等位基因频率分布差异有显著性的意义 (P <0 .0 5)。而TNF βG/G、G/A和A/A基因型在病例组各占 3 0 %、40 %和 3 0 % ,对照组中分别为 2 6%、 51%和 2 3 % ,二组比较差异无显著性 (P >0 .0 5)。结论 :TNF αG等位基因可能是乙型肝炎易感性的遗传标记 ;TNF αG

To assess the possible association between the polymo rphisms of tumor necrosis factor (TNF)gene and the susceptibility of HBV infec tion.Method: TNF α and TNF β genotypes were detected with the met hods of PCR RFLP in case control study including 146 cases of HBV hepatitis an d 165 healthy controls.Results: The distributions of G/G and G/A genotypes in the TNF α promoter region at positions -308 were 95% and 5% in cases of HBV hepatiti s,whereas 88% and 12% in the controls,The frequency of TNF α G allele and dist ributions of TNF α genotype in cases of HBV hepatitis were significantly diffe rent from those of the controls( P <0.05).The distributions of G/G,G/A and A /A genotypes in the TNF β intron 1 at position +252 were 30%,40% and 30% in ca ses of HBV hepatitis,26%,51% and 23% in the controls,respectively.There was no a ssociation between HBV hepatitis patients and any allele or genotype of TNF β polymorphisms.Conclusion: TNF α G allele may be one of the susceptibly genet ic markers of HBV infection in Han′s population of China.The risk of HBV infect ion can be increased in person with TNF G/G genotype.