摘要
目的:探讨用抗体封闭神经干细胞膜受体的效果,以建立一种研究神经生长因子或神经营养因子对神经干细胞作用的实验方法。方法:用抗表皮生长因子受体多抗和二抗封闭神经干细胞的表皮生长因子受体。结果:神经干细胞表皮生长因子受体被封闭,并且因失去表皮生长因子刺激而死亡。结论:抗体封闭膜受体的方法在研究神经生长因子或神经营养因子对神经干细胞作用的体外实验中有一定价值。
Objective: To observe the effect of antibody blocking NSC membrane receptor in order to develop a new experimental method for researching the effect of growth factors and neurotrophic factors upon NSC. Methods: Block NSC EGFR with anti-EGFR polyclonal antibody and corresponding second antibody. Results: the NSC EGFR was blocked, and the NSC died of lack of EGF. Conclusion: It is of some worth using antibody to block membrane receptor for in vitro researching the effect of growth factors and neurothophic factors upon NSC.
出处
《黑龙江医药科学》
2002年第5期8-9,共2页
Heilongjiang Medicine and Pharmacy
基金
国家自然科学基金资助项目(批准号30170963)
