二种抗原检测结核分枝杆菌抗体的临床意义评价

Evaluation of two TB antigens for detecting of anti-TB-antibodies by DIGFA

目的 评价两种抗原检测结核分枝杆菌抗体对结核病诊断的意义。方法 以结核分枝杆菌多肽 (TB4 )和脂阿拉伯甘露糖 (LAM )为抗原 ,斑点金免疫渗滤法为检测方法对 78例临床确诊为肺TB的病人血清、15例气管炎患者血清和 5 0例正常人血清进行抗体测定 ,观察这些抗原诊断结核病的敏感性、特异性。结果 两种抗原检测结核抗体的敏感性分别为84 6 % (6 6 / 78)和 73 1% (5 7/ 78) ;特异性分别达 92 3% (6 0 / 6 5 )和 95 4 % (6 2 / 6 5 ) ,与痰涂片的符合率为 84 8%和 81 8%。各指标比较 ,经 χ2 检验表明 ,差异无显著性意义 (P >0 0 5 )。结论 提示TB4是一种敏感性高 ,特异性强的抗原 ,可用于临床查病和血清流行病学调查。

Aim To evaluate the clinical value of two TB antigens for detecting serum anti TB antibodies in diagnosis of tuberculosis Methods Polypeptide(TB 4)and Lipoarabinomannan(LAM)of Mycobacterium tuberculosis(TB)were used as antigens to detect serum anti TB antibodies by dot immunogold filtration assay(DIGFA) Sensitivity and specificity of two antigens in diagnosis of TB were detected 78 sera from patients infected with TB,15 sera from patients with tracheitis and 50 sera from normal adults were tested using two antigens by DIGFA Results Sensitivity of two antigens in detecting anti TB antibodies were 84 6%(66/78)and 73 1%(57/78),specificity of them were 92 3%(60/65)and 95 4%(62/65)respectively Coincidental rate with sputum smear were 81 8% and 84 8% respectively There is no significant difference between TB 4 and LAM antigens(P>0 05) Conclusion It suggests that TB 4 is an available antigen with high sensitivity and specificity for detecting anti TB antibody by DIGFA It is fitful to clinical diagnosis and epidemiological investigation