应用胶原支架构建组织工程心脏瓣膜的初步研究

In vivo experience with tissue engineered heart valve on collagen scaffold

目的 探讨应用胶原支架构建组织工程心脏瓣膜的可行性。方法 行兔皮下包埋实验 ,分别于 4、6、8、12周观察材料的生物相容性和降解率。将犬主动脉壁间质细胞和主动脉内皮细胞种植于胶原膜上 ,进而将此瓣叶植入犬腹主动脉内 3个月 ,观察材料吸收和瓣叶结构的变化。结果 胶原膜呈网孔状结构 ,孔径 10 7μm。皮下包埋实验显示 ,材料生物相容性好 ,完全降解时间为 12周。体内实验显示 ,材料于 12周末被间质细胞合成的细胞外基质所取代 ,瓣叶表面有内皮细胞覆盖。结论 以胶原膜为支架构建组织工程心脏瓣膜是可行的 ,但材料在强度和组织相容性方面尚需改进。

Objective: To evaluate the feasibility of tissue engineered heart valve on collagen scaffold. Methods: To observe constructive characteristics of collagen scaffold with scanning electron microscope. Collagen membrane was implanted subcutaneously in rabbit and extirpated at 4, 6, 8, 10 weeks in order to study its biocompatibility and biodegradibility. The collagen scaffold were seeded with the cells of aortic wall and aortic endothelium of canine, and implanted in the abdominal aorta to assess the reconstruction of the valve leaflet. Results: Porous diameter of collagen scaffold is 107 μm. Collagen scaffold has good biocompatibility and is completely degraded at the end of the 12th week. The scaffold was completely substituted with the extracellular matrix secreted by the seeded cells at the end of 12th week in vivo. SEM showed valve leaflets were covered with endothelial cells. Conclusion: It is feasible to construct an tissue engineered heart valve in vivo on collagen scaffold.