摘要
本文采用银杏果实和植株诱发的茎尖和叶尖为原料,筛选银杏愈伤组织生长和继代培养的最佳条件和激素组合;利用继代培养愈伤组织进行细胞悬浮培养并检测黄酮的生物合成量。结果表明:（1）银杏愈伤组织的诱导及继代培养以附加NAA3.0mg/L和BA1.0mg/L的MS培养基效果最好;（2）采用0.1％浓度的植酸可以抑制褐变,并对愈伤组织的生长有促进作用;（3）在培养基中添加5％的蔗糖时愈伤组织增长倍数最高;愈伤组织继代培养40～45d时增长倍数达到最大值;（4）银杏愈伤组织细胞悬浮培养时,细胞生长速度可达6.8倍,黄酮生物合成量为68.76mg/L。
Seed and young stem as well as young leaf of Ginkgo biloba were used to induce callus. Optimum conditions and hormone combinations were sifted for Ginkgo callus growth and subculture. The biosynthesis amount of flavonoids was assayed in the callus suspension culture. The results showed: (1) The culture medium for induced and subculture of Gingko biloba callus was the MS culture medium by adding 3. 0mg/L NAA and 1. 0 mg/LBA. (2)0. 1% phytic acid has inhibited the callus browning and promoted the callus growth. (3) Adding 5. 0% of sucrose in culture medium increased the callus growth and the growth time of the callus reached amaximum value during subculture 40 to 45 days. (4) Gingko callus growth rate was 6. 8 times higher during suspension culture and biosynthesis amount of the flavonoids reached 76 mg/L.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第11期38-41,共4页
Food Science
