摘要
通过单因子试验和正交表试验,对黑曲霉（As.n.XD-1）β-葡萄糖苷酶（EC.3.2.1.21）产酶条件进行了研究。试验表明:黑曲霉（As.n.XD-1）产β-葡萄糖苷酶适宜于固体发酵,适宜条件为甘蔗渣与麦麸配比为2:3,酵母膏0.8％,加水比为1:3,初始pH值自然,于28℃发酵4d。在这个优化条件下酶活力达23.86U/ml。
β - glucosidase (EC. 3.2. 1.21) production conditions of Aspergillus niger (As. n. XD - 1) were investigated through single factor experimentation and orthogonal test. The results showed that solid state fermentation was the suitable way of β - glucosidase production were 2 portions of bagasse, 3 portions of wheat bran, 0. 8% yeast extract, 3 times of water, initial natural pH value, and fermentation at 28() for 4d. Under these conditions, the activity of β - glucosidase could reached 23. 86U/ml.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第11期59-62,共4页
Food Science
基金
国家自然科学基金(20066001)
