摘要
本实验采用差速离心、脱氧胆酸钠增溶、DEAE-纤维素和Sephadex G-200层析等方法,从兔肺中分离提取血管紧张素转换酶。通过研究表明:它的分子量为302KD,含糖量为19.3%,最适温度和pH值分别为37℃和8.3。
Angiotensin - converting enzyme was extracted by differential centrifugation, the solubilization of sodium deoxycholate, DEAE - cellulose column chromatography and Sephadex G - 200 column gel filtration. A series of studies on angiotensin - converting enzyme indicated that it had molecular weight of 302KD, the sugar content was 19. 3% and the optimum temperature and pH were 37℃ and 8. 3 respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2002年第11期126-128,共3页
Food Science