Rhenium labeled McAb-SZ39 and colloid

Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared.Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186 ReO4^-.When ascorbic acid was used as reduction agent of antibody and the reduction of 186 ReO4^- was finished in sodium gluconate solution(pH=2.54),the labeling efficiency was 96.2%,After purification,the labeled products were tested in respect of immunoreactivity,in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice,188ReO4^- labeled colloid was synthesized by using a two-step method.The size of radiocolloid particles was around 2-5μm,and the radiochemical yield was over 95%,Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-collied and ^188 Re-McAb-SZ39 was 90% and 65% ,respectively.

Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared. Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with 186ReO4-. When ascorbic acid was used as reduction agent of antibody and the reduction of 186ReO4- was finished in sodium gluconate solution (pH=2.54), the labeling efficiency was 96.2％. After purification, the labeled products were tested in respect of irnmunoreactivity, in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice. 188ReO4-labeled colloid was synthesized by using a two-step method. The size of radiocolloid particles was around 2~5/mi, and the radiochemical yield was over 95％. Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of 188Re-colloid and 188Re-McAb-SZ39 was 90％ and 65％, respectively.