STIMULATING EFFECT OF LOW DOSE ^(147)Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

ＳＴＩＭＵＬＡＴＩＮＧＥＦＦＥＣＴＯＦＬＯＷＤＯＳＥ^（１４７）ＰｍＯＮＤＮＡＲＥＰＡＩＲＩＮＳＰＥＲＭＩＯＧＥＮＩＣＳＴＡＧＥＳＳＴＩＭＵＬＡＴＩＮＧＥＦＦＥＣＴＯＦＬＯＷＤＯＳＥ^（１４７）ＰｍＯＮＤＮＡＲＥＰＡＩＲＩＮＳＰＥＲＭＩＯＧＥＮＩＣＳＴ...

For labelling spermatid DNA, male BALB/c mice are injected intratesticularly with 3H-TdR in advance. After 36 d of labelling, spermatozoa are sampled which is just the time required for the germ cells to mature. The period from intraperitoneal injection of 147Pm to sampling spermatozoa is 12d. After sperm cells have been lysed, 30ml eluent is added by wriggle pump. Finally, both the DNA remained on the filters and filtrated bottles are determined with a Beckman liquid scintillation device. Results show that after small dose internal irradiation with 185 Bq/g of 147Pm, an increase in amount of sperm DNA on the filter observed is considerably higher than the control group. This indicates that low level internal radiation of 147Pm has tendency to stimulate the DNA repair in spermiogenic stages.