人胚胎关节软骨细胞库的建立

Establishing bank of human embryo articular cartilage chondrocytes

目的体外建立人胚胎关节软骨细胞冻存、复苏的稳定技术,为软骨组织工程提供大量的、具有软骨细胞生物活性的种子细胞。方法将传代培养的人胚胎关节软骨细胞用含10%DMSO、50%胎牛血清的IMDM培养液悬浮后,置于-196℃的液氮中长期保存,建立人胚胎关节软骨细胞库。然后将细胞库中的冻存细胞进行复苏培养,观察其生长状况,并测定软骨细胞中DNA及糖醛酸的含量。结果冻存软骨细胞复苏后仍保持旺盛的增殖与分泌细胞外基质的功能。结论关节软骨细胞库的建立,可以长期保存培养的软骨细胞。

Objective To provide large amount of seed cells which maintain the biological chara cteristic of normal chondro-cytes by establishing stable technology of cryopreservation of human em bryo articular cartilage chondrocytes in -vitro.Methods Subcultured human embryo articular cartilage chondrocytes were preserved in liquid nitrogen(-196℃)after they were suspen-sioned in IMDM medium with 10%DMSO an d 50%fetal bovin serum.The biologic al characteristic was observed and t he content of DNA and Uronic acid of cultured cry opreserved chondrocytes examined.Results Cultured cryopreserved chondrocytes maintain the ability to proliferate and to secrete extra cellular matrix.Conclusions Establishing bank of human embryo articular cartilage chondrocytes could preserve cultur ed chondrocytes over a long period.