细胞凋亡相关基因表达与乳腺癌预后的关系

The relationship between the apoptosis related genes and the prognosis of breast cancer

目的 :研究乳腺癌中凋亡相关基因Bcl 2、P53的表达及其组合与影响预后的因素 (肿瘤大小、淋巴结转移、PCNA、ER和PR)之间的关系。方法 :采用免疫组化SP法检测 54例乳腺癌标本。结果 :Bcl 2和P53表达阳性率分别为 63 .0 %和 40 .7%。Bcl 2高表达与肿瘤体积小 ,肿瘤增殖活性低 ,ER、PR阳性显著相关 ;P53高表达与肿瘤增殖活性高 ,ER、PR阴性显著相关 (P <0 .0 5或P <0 .0 1 )。Bcl 2与P53表达之间呈显著负相关 (P <0 .0 1 )。Bcl 2 + /P53- 较Bcl 2 - /P53+ 肿瘤体积小 ,肿瘤增殖活性低 ,ER、PR阳性率高 (P <0 .0 5或P<0 .0 1 )。Bcl 2 + /P53+ 较Bcl 2 - /P53+ 肿瘤体积小 ,PR阳性率高 (P <0 .0 5)。结论 :Bcl 2高表达、P53低表达与乳腺癌好的预后因素相关。预后以Bcl 2 + /P53- 为最佳 ,Bcl 2 - /P53+ 最差 ,Bcl 2 + /P53+ 似较Bcl 2 - /P53-佳。

Objective: To study the relationship between the expression of P53,Bcl-2 and the factors o f prognosis of breast cancer. Methods:54 cases of breast cancer were studied by using SP immu nohistochemical technique.Results: The overall positive rates of Bcl-2 and P53 expression were 63.0% and 40.7%. T he high expression of Bcl-2 coincided with those good prognostic factors such a s small tumor size,low label index of PCNA(LI),the presence of ER and PR(P<0.05 or P<0.01). The high expression of P53 coincide d with those bad prognostic factors such as high LI,the absence of ER and PR(P<0.05 or P<0.01). There was a significant negativ e correlation between the expression of Bcl-2 and that of P53(P <0.01). The volume was smaller,the LI was lower and the positive rate of ER and PR was higher,with Bcl-2 +/P53 - than the ones with Bcl-2 -/P53 +(P<0.05 or P<0.01). Conclusion: High expression of Bcl-2 and low expression of P53 are the good prognostic fa ctors of breast cancer. The phonotype of Bcl-2 +/P53 - indicates the best pro gnosis while Bcl-2 -/P53 + indicates the poorest prognosis.