用抗血小板膜糖蛋白ⅡbⅢa单克隆抗体检测ITP自身抗体的临床应用

MEASURED THE AUTOANTIBODIES IN ITP WITH MONOCLONAL ANTIBODIES (AGAINST GP Ⅱb,G P Ⅱa) AND ITS APPLLCATION

本文利用抗血小板膜糖蛋白Ⅱ、b、Ⅲa(GPⅡb、GPⅢa)单克隆抗体,采取酶联免疫双抗体夹心法分别检测了26例特发性血小板减少性紫癜(ITP)患者血浆抗GPⅡb、GPⅢa自身抗体.结果显示3例抗GPⅡb阳性(约占11.5%),1例抗GPⅢa(约占3.8%),7例同时抗GPⅡb.GPⅢa(约占26.9%),阳性总数为11例(约占42.3%);且抗GPⅡb. GPⅢa阳性结果与血小板数无关.

By enzyme-linked immunosorbent assay (ELISA), were measured the autoantibodies to the GPⅡb, GPⅡa in the 30 normal sublects anb 17 secondary thrombocytopemia, 26 idiopathic thrombocytopenia purpura(ITP) using monoclonal antibodies SZ - 21 (against GpⅢa), SZ - 22 (against Gp Ⅲ b). these tests showed: (1) 7 cases gave positive results of SZ-21 and SZ-22(26.9%), 1 case only gave Positive result of SZ-22 (3.8%), 3 cases only gave Positive results of SZ-21 (11.5%); the Positive ratio was about 42.3%. (2) the SZ-21, SZ -22 walues in the ITP plasma were nor correlated with platelet count. These data suggesting that this approach may be useful in studying the diagnoses and pothogenesis of ITP.