冠心病患者载脂蛋白AI基因多态性的研究

Studies on the association of apolipoprotein AI gene polymorphism with coronary heart disease

目的 探讨载脂蛋白 AI (Apo AI)基因多态性与冠心病的关系。方法 经冠状动脉造影选择对照组(1 82例 )和冠心病组 (4 62例 ) ,应用 PCR,Msp I内切酶消化及 PAGE的方法确定 Apo AI基因多态性。结果 冠心病组和对照组均以 M1 + + 、M2 + + 基因型最常见 ,M1 + - 次之 ,两组比较均无显著性差异 (P>0 .0 5 )。冠心病组 M1 - - 、M2 + -基因型频率明显高于对照组 ,并且随着病变冠脉的数量增加而增加 (P<0 .0 2 5～ 0 .0 0 5 )。各冠心病亚组中 ,两种 Apo AI基因多态性并存的频率高于对照组 ,3支病变组与对照组、 1支病变组比较 ,差异有显著性 (P<0 .0 5～ 0 .0 2 5 )。结论 Apo

Objective To explore the association of apolipoprotein AI(ApoAI)gene polymorphism with coronary heart disease(CHD).Methods Four hundred and Sixty two patients with CHD and 182 non CHD as controls that were determined by coronary angiography.Polymerase chain reaction(PCR),MspI digestion,polyacrylamide gel electrophoresis,and silver staining were used for the detection of ApoAI genotype.Results The M1 ++ and M2 ++ genotypes were most common in patients with CHD and controls.There were no differences between two groups(p>0.05).The frequencies of the M1 -,M2 +- genotypes in CHD group were significantly higher than those of control group(p<0.025～0.005),and they increased linearly with the number of diseased vessels.The frequency of combination with two polymorphism(M1 +- ,M1 -- and M2 +- )in CHD subgroups was higher than that of controls.Patients with three diseased vessels had significantly higher frequency of combination of two polymorphism than those with no diseased vessel and single vessel(p<0 05～0 025).Conclusions These results suggest that ApoAI gene polymorphism is involved in the occurrence and the severity of CHD.