载脂蛋白E基因多态性与脑血管病的关系

The relationship between polymorphisms of apolipoprotein E gene and cerebrovascular disorder

目的 探讨载脂蛋白 E ( APOE)基因多态性与脑血管病 ( CVD)的关系。方法 应用聚合酶链反应—限制性长度多态性技术 ( PCR-RFLP) ,检测 30例脑出血 ( CH)及 6 3例脑梗死 ( CI)患者的 APOE基因型 ,并与 6 6名同龄健康对照组比较。结果 CH组 APOE基因型ε4及ε4携带者的频率明显高于对照组 ( P<0 .0 5 ) ,ε3及ε3/3频率显著低于对照组 ( P<0 .0 1 ) ;CI组 APOE基因型 ε3/4明显高于对照组 ( P<0 .0 5 )。结论 APOE基因 ε3/4与 CI的发生相关 ,等位基因 ε4可能是 CVD的一种遗传易感因子 ,ε3/3可能与 CVD的保护有关。

Objective To study a relationship between the polymorphisms of apolipoprotein E(APOE) gene and Cerebrovascular disorder(CVD).Methods The polymerase chain reation(PCR)and restriction fragment length polymorphism(RFLP)technique were used to detect APOE genotype in 30 Cerebral hemorrhage(CH),63 Cerebral infarction(CI)patients and 66 age matched controls.Results The frequency of genotype ε4 and ε4 carrier in the CH group was significantly higher(P<0.05),whereas ε3 and ε3/3 was significantly lower(P<0.01),and ε3/4 in the CI group was significantly higher(P<0.05)when compared with controls,respectively. Conclusions APOE genotype ε3/4 might be responsible for the development of CI and allele ε4 seemed to be a genetic susceptible factor. On the contrast,ε3/3 might be related to the protection of CVD.