低相对分子质量肝素对牛晶状体上皮细胞在人工晶状体表面增殖的影响

Influence of low molecular weight heparin on proliferation of bovine lens epithelial cells on the surface of intraocular lens

目的 体外比较研究低相对分子质量肝素 (lowmolecularweightheparin ,LMWH)和普通肝素影响牛晶状体上皮细胞 (bovinelensepithelialcell,BLEC)在聚甲基丙烯酸甲酯 (polymethylmethacrylate,PMMA)人工晶状体表面增殖的作用。方法 体外将BLEC接种到PMMA人工晶状体表面培养 ,LMWH组和肝素组培养液中分别含LMWH(1× 10 5U/L)和普通肝素 (1× 10 5U/L) ,对照组培养液中不含上述药物。 72h后用流式细胞仪对人工晶状体表面黏附的细胞进行计数分析。结果 经LMWH和普通肝素处理的PMMA人工晶状体表面BLEC形态幼稚 ,对照组BLEC呈纤维化趋势 ;细胞计数对照组为(878 2± 10 7 8)个 /ml,肝素组为 (5 4 5 5± 5 8 2 )个 /ml,LMWH组为 (40 7 8± 36 6 )个 /ml,3个组比较差异有非常显著意义 (P <0 0 0 1)。结论 LMWH可显著抑制晶状体上皮细胞在人工晶状体表面的黏附和增殖 ,有望成为更为安全。

Objective To investigate the anti proliferation effect of low molecular weight heparin (LMWH) on bovine lens epithelial cells (BLEC) on the surface of polymethylmethacrylate (PMMA) intraocular lens (IOL) Methods BLECs were incubated in vitro on the surface of PMMA IOLs The cultural media contained LMWH (1×10 5 U/L) or normal heparin (1×10 5 U/L) or none of them (control) Cells were counted by flow cytometry Results The heparin or LMWH treated BLECs on the surface of PMMA IOLs showed immature appearance, while the BLECs in the control group showed a tendency of fibrosis The numbers of BLECs (per ml) in the digested solution from the surface of IOLs were 878 2±107 8 (control), 545 5±58 2 (heparin) and 407 8±36 6 (LMWH) The comparisons among the three groups showed significant differences ( P <0 001). Conclusion Low molecular weight heparin inhibits proliferation of lens epithelial cells on the surface of intraocular lens, which could be used as a potential drug to prevent the occurrence of postoperative capsular opacification after IOL implantation