脂肪酸影响血管内皮细胞纤溶酶原激活物抑制剂-1表达的机制初探

The mechanism-exploring of fatty acids influencing plasminogen activator inhibitor -1 expression in endothelial cells

目的 探讨脂肪酸影响血管内皮细胞纤溶酶原激活物抑制剂 1表达的机制。方法 以PAI 1启动子控制表达氯霉素转移乙酰酶 (CAT)报告基因的重组质粒—PAI pCAT转染人血管内皮细胞株ECV30 4,并且部分共转染不同量的过氧化体增殖物激活型受体 (PPAR)α或PPARγ表达载体。分别以亚麻酸、亚油酸、油酸、硬脂酸诱导转染细胞 ,酶联免疫吸附方法测定CAT表达量显示启动子片段转录活性。结果 亚麻酸、亚油酸、油酸诱导以及增加PPARα表达可提高PAI 1启动子转录活性 ,硬脂酸诱导和增加PPARγ表达无影响。结论 不饱和脂肪酸可通过提高PAI 1转录活性诱导其在血管内皮细胞的表达 ,此作用涉及PPARα对PAI 1基因转录的诱导调节。

Objective To investigate the mechanism of various fatty acids′ effect on plasminogen activator inhibitor -1 expression in human endothelial cells.Methods Cultured endothelial cell line-ECV304 was co-transfected with PAI-1 promoter controlled CAT reporter gene, and PPARα or PPARγ expression vetors. These cells were exposed to lionlenic acid, linoleic acid, oleic acid and stearic acid. The transcripton activity of PAI-1 promote was demonstrated as CAT expression termined by ELISA. Results Induction of lionlenic acid,linoleic acid, oleic acid and enhancing expression level of PPARα could increased transcription activity of PAI-1 promoter, while induction of stearic acid and enhancing espression of PPARγ had no major effect. Conclusion The unsaturated fatty acids can induce the expression of PAI-1 through promoting transcription activity of PAI-1 promoter in endothelial cells. PPARα plays an active role in this effect.