摘要
研究内源性单胺氧化酶B抑制因子 靛红 (2 ,3 吲哚醌 ,isatin)对MPTP引起的小鼠纹状体DA含量降低的影响。用高效液相色谱配电化学检测器测定纹状体DA ,DOPAC和HVA水平。MPTP 30mg/kgip使纹状体DA ,DOPAC和HVA含量与各自对照组比较显著降低 (P <0 .0 1) ,预先靛红 (2 0 0mg/kg·d ,× 4d ,ig)几乎完全抑制了上述效应 ,并减少了MPTP对DA更新率的升高 ,说明靛红有预防MPTP对中枢DA能神经元的毒性。
The present study was designed to examine the effects of isatin(indole 2,3 dione), an endogenous selective monoamine oxidase type B(MAO B) inhibitor, on the 1 methyl 4 phenyl 1,2,3,6 tetrahydropyridine (MPTP) induced reduction of dopamine (DA) and its metabolites in striatum of C57BL/6J mice. The amount of DA, dihydroxy phenylacetic acid (DOPAC) and homovanillic acid (HVA) in striatum of C57BL/6J mice was measured by high performance liquid chromatography coupled with electrochemical detection. It was found that, administration of MPTP (30 mg/kg, ip) caused significant decrease of striatal DA,DOPAC and HVA ( P <0.01, compared with the control group) , while isatin (200 mg/kg·d,×4d, ig) almost completely inhibited the MPTP induced reduction of these parameters in the striatum, and reduced the increase of DA turnover induced by MPTP. In conclusion, isatin is able to prevent the depletive action of MPTP on central dopaminergic neurons, and therefore exerts a protective effect on the central dopaminergic system.
基金
国家自然科学基金 (3 0 0 76867)
国家卫生部基金 (96 2 3 2 3 )资助项目
