腹主动脉瘤信号传导相关基因的研究

Studies on the differential expression of signal transduction related gene in human abdominal aortic aneurysms

目的研究细胞信号传导基因在腹主动脉瘤和正常主动脉的差异表达 ,探讨其与腹主动脉瘤发生的关系。方法应用基因芯片技术检测腹主动脉瘤和正常主动脉信号传导相关基因的表达 ,筛选出差异表达显著的基因 ,从RNA和蛋白质水平作进一步的分子生物学实验。结果发现腹主动脉瘤差异表达的信号传导基因 45条 ,在动脉瘤组织中表达上调基因为 2 8条 ,表达下调基因 17条。对其中MAPK信号系统的进一步实验发现ASK1、ERK1等基因异常表达 ,均在主动脉中层有蛋白质表达。

ObjectiveTo study the differential expression of signal transduction related gene between the tunica media in human abdominal aortic aneurysms (AAA) and normal aorta, and inspect their role in the etiology of abdominal aortic aneurysms.MethodsGene chip technique was applied to study the differential expression of signal transduction related gene in human AAA, then screening some significant differential expressed genes for further research by RT-PCR, Western Blot, and immunohistochemistry.ResultsForty-five differentially expressed genes related to cellular signal transduction were detected accounting for 1.09% of the chip′s total 4?096 genes,among them 28 genes were up-regulated and 17 down-regulated in aortic aneurysm. ASK1, ERK1 gene were found up-requlated in aortic aneurysm.ConclusionsDifferential expression of signal transduction gene could play a key role in the occurrence of AAA.